Coherent Anti-Stokes Raman Scattering Cell Imaging and Segmentation with Unsupervised Data Analysis

Offering the high spectral resolution of conventional Raman spectroscopy combined with reduced acquisition time, multiplex coherent anti-Stokes scattering (MCARS) microspectroscopy using sub-nanosecond laser pulses has been accepted as a mature and straightforward technology for label-free bioimaging. In recently published paper, Philippe Leproux associates have introduced combination MCARS imaging technique unsupervised data analysis based on multivariate curve (MCR). spoke to Spectroscopy about combining techniques, well discussing potential results.